Knowledge Base

O8) Tandem Lens Macroscope configuration

Tags: DoLP, Front coupled lenses, High Numerical Aperture, In vivo cortex imaging, Intrinsic Imaging, Low magnification high NA, Macroscope, Optical Imaging of Intrinsic Signals in the Cortex, Optical Inspection, Polarized Light inspection, tandem lens, Voltage sensitive dyes, Widefield fluorescence macroscope

O7) How to choose a compatible lens (an example)

Tags: Choosing a Lens, Field of View, Focal Length, Line pairs per mm, Nyquist limited pixel size, Optical Resolution, Working Distance

O6) Choosing a Lens to meet FOV and WD requirements

Tags: Angular Field of View, Choosing a Lens, Field of View, Field of View Equations, Focal Length, Minimum Working Distance, Working Distance

O5) Optical Resolution of a Camera and Lens System

Tags: Diffraction, Diffraction Limited resolution, f/# of a lens, Limiting Resolution, Line pairs per mm, Numerical Aperture, Nyquist Criteria, Optical Resolution

O4) Lens Iris/Aperture setting (f/#)

Tags: f/# of a lens, f# and Depth of Field, Fast Lenses, Light Collection and f/#, Numerical Aperture and f/#

O3) Camera Lens fundamentals

Tags: Angular Field of View, Field of View, Focal Length, Lens Specifications, Optical Format, Optical Magnification, Vignetting, Working Distance

O2) Lens Selection

Tags: Field of View, Optical requirements, Smallest resolvable feature, Working Distance, Zoom lens

O1) Welcome to our Optics Knowledge Base

M10) Super Resolution Fluorescence Microscopy

Tags: Dr. David Agard, Dr. Jennifer Lippincott-Schwartz, Dr. Stefan Hell, Photo Activated Localization Microscopy (PALM), Prof. Xiaowei Zhuang, Reversible Saturable Optical Fluorescence Transitions (RESOLFT), sCMOS cameras, Stimulated Emission Depletion (STED), Stochastic Optical Reconstruction Microscopy (STORM), Structured Illumination Microscopy (SIM), Super Resolution Microscopy

M9) Advanced Techniques in Fluorescence Microscopy

Tags: Dr. Daniel Axelrod, Dr. Ernst Stelzer, Dr. Hari Shroff, Dr. Jennifer Lippincott-Schwartz, Dr. Phillipe Bastiaens, Dual-view Inverted Selective Plane Illumination (diSPIM), Fluorescence Loss in Photobleaching (FLIP), Fluorescence Recovery After Photobleaching (FRAP), Forster Resonance Energy Transfer (FRET), Light Sheet Microscopy, sCMOS cameras, Selective Plane Illumination Microscopy (SPIM), Total Internal Fluorescence Microscopy (TIRF)