Knowledge Base

M8) Microscopy Techniques and Camera Selection (Fluorescence)

Tags: Dr. Jennifer Waters, Dr. Nico Stuurman, Dr. Roger Tsien, Filter Selection for Fluorescence Microscopy, Fluorescence Microscopy, Green Fluorescent Protein (GFP)

M7) Microscopy Techniques and Camera Selection (Transmitted Light)

Tags: Birefringence, cmos cameras, Dark Field Microscopy, Differential Interference Contrast (DIC), Dr. Shinya Inoue, Phase Contrast Microscopy, Polarized Light Microscopy, Prof. Edward Salmon

M6) How to select the right microscopy technique

Tags: Dark Field Microscopy, Deconvolution, Dr. Nico Stuurman, Epifluorescence, Fluorescence Microscopy, Micro-manager software, Phase Contrast Microscopy, Prof. Ron Vale, sCMOS cameras, Selecting the right microscopy technique, Spinning Disk Confocal Microscopy, STED, STORM/PALM, Super Resolution Microscopy, Total Internal Reflection Fluorescence (TIRF)

M5) Camera selection in Microscopy, based on FOV and resolution

Tags: cmos cameras, Estimating FOV, Estimating Optical Resolution, High-magnification objectives, Low-magnification objectives, Magnification, Numerical Aperture, sCMOS cameras, Selection Criteria

M4) How Diffraction Limits the Optical Resolution of a Microscope

Tags: Axial and Lateral Resolution Limits in a Light Microscope, Diffraction, Dr. Bo Huang, Dr. Jennifer Waters, Dr. Kurt Thorn, Experimental demonstration of the Fourier Plane in a Light Microscope, Numerical Aperture, Nyquist Criterion, Point Spread Function, Prof. Jeff Lichtman, Rayleigh Criterion, Resolution Limit of a Light Microscope

M3) Optical Fundamentals in Light Microscopy

Tags: Dr. Jennifer Waters, Dr. Stephen Ross, Image formation in a Microscope, Koehler illumination, Lenses and Image Formation, Microscope Objectives, Numerical Aperture, Point Spread Function, Prof. Daniel Fletcher, Prof. Ron Vale

M2) Introduction to Light Microscopy

Tags: Advances in Light Microscopy, Dr. Shinya Inoue, History of Microscopy, Prof. Joseph Gall, Prof. Ron Vale, What can you learn with a Light Microscope?

M1) Welcome to our Microscopy Knowledge Base